APPLICATION OF PCR FOR THE DETECTION OF TUBERCLE BACILLI FROM RETROPHARYNGEAL AND THYROID ABSCESS: CASE REPORTS

  • Narayan Raj Bhattarai B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Keshav Rai B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Ratna Baral B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Shyam Thapa Chettri B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Deepak Paudel B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Smriti Karki B.P. Koirala Institute of Health Sciences, Dharan, Nepal
  • Basudha Khanal B.P. Koirala Institute of Health Sciences, Dharan, Nepal
Keywords: Extra-pulmonary tuberculosis, IS6110, M. tuberculosis, PCR

Abstract

The diagnosis of extra-pulmonary tuberculosis by Mycobacterium tuberculosis infection in retropharyngeal and thyroid abscess is challenging due to a lack of rapid, sensitive and specific diagnostic assay. Hence, we evaluated the performance of highly sensitive polymerase chain reaction (PCR) to detect the M. tuberculosis in the retropharyngeal and thyoid abcess. The microscopy and culture were negative test result however, PCR result had demonstrated the presence of M. tuberculosis in the same specimens with retropharyngeal and thyroid abscess. Therefore, it is recommended to perform PCR to detect undiagnosed cases, which were not detected by conventional approaches for the diagnosis, of extra-pulmonary tuberculosis.

Author Biographies

Narayan Raj Bhattarai, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Microbiology, Additional Professor

Keshav Rai, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Microbiology, Senior Demonstrator

Ratna Baral, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Microbiology, Additional Professor

Shyam Thapa Chettri, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Otolaryngology, Professor

Deepak Paudel, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Otolaryngology, Associate Professor

Smriti Karki, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Pathology, Professor

Basudha Khanal, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

Department of Microbiology, Professor

Published
2019-12-27
Section
Case Reports